학술포스터 목록

번호	구분	연도	제목	파일	작성일	조회
318		2023	작물기초기반과 최수연 Peanut (Arachis hypogaea L.) is one of the widely cultivated crops in the world and is also popular as health food because they contain good sourse of vitamins and fiber. In Korea, peanut is repeatably cultivated in Jeju-si, Yeoju-si and Gochang-gun. Peanut is vulnerable by soil pathogens until harvest because pods grow underground. In July 2022, symptoms of wilting aboveground were found in Yeoju-si and Wanju-gun. When the collected collars of peanuts were cut, it was confirmed that the vessel browned. Surface-sterilized soil- surfaces were plated on PDA medium and incubated at 25℃. Genomic DNA was extracted from three isolated strains (YJ1-2, YJ3-2 and JJ2-1), and was amplified using ITS4 and ITS5 primer set. Based on the ITS gene sequences, three strains were confirmed to highly similarity to Pythium myriotylum with 99%. To substantiate the athogenicity of strains, peanut seeds and P. myriotylum three strains were co-cultured on water agar to observe root growth. After eight days, root length measurements showed that all three strains inhibited peanut root growth. Also, the same symptom as in the field was observed when peanut plants were inoculated with P. myriotylum. This is the first report of peanut wilting disease caused by P. myriotylum on peanut in Korea.  " target="_blank" title="Occurrence of Peanut Wilting Disease Caused by Pythium myriotylum in Korea 새창에서 열림"> Occurrence of Peanut Wilting Disease Caused by Pythium myriotylum in Korea		2024.02.13	25
317		2023	작물기초기반과 송승엽 들깨는 꿀풀과에 속하는 유료 작물 중 하나로 생육기간이 짧고, 재배지 토 양에 대한 적응성이 높아 혼작과 윤작이 가능한 주요 작물로 평가된다. 또 한 루테올린, 로즈마릭산, 폴리코사놀 등의 다양한 기능성 물질을 함유하고 있어 건강 기능성 식품으로의 주목도가 높아지고 있다. 이에 국내에서 재 배되고 있는 들깨 품종을 육종 소재 탐색 및 들깨 품종 육성의 기반을 마련 하고자 들깨 기능성 유용대사체를 분석하였다.  " target="_blank" title="들깨 기능성 유용 대사체 분석 새창에서 열림"> 들깨 기능성 유용 대사체 분석		2024.02.13	17
316		2023	작물기초기반과 송승엽 To determine whether FT-IR spectral analysis based on multivariate analysis for whole cell extracts can be used to discriminate between Soybean plants seed at the metabolic level, leaves of 383 soybean core collection plants were subjected to Fourier transform infrared(FT-IR) spectroscopy. FT-IR spectral data from leaves were analyzed by principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA). FT-IR spectra confirmed typical spectral differences between the frequency regions of 1,700 - 1,500, 1,500 - 1,300 and 1,100 - 950 cm-1, respectively. These spectral regions reflect the quantitative and qualitative variations of amide I, II from amino acids and proteins (1,700 - 1,500cm-1), phosphodiester groups from nucleic acid and phospholipid (1,500 - 1,300cm-1) and carbohydrate compounds (1,100 - 950cm-1). PCA revealed separate clusters that corresponded to their species relationship. Thus, PCA could be used to distinguish between soybean with different metabolite contents. PLS-DA showed similar metabolite contents of soybean. Further more these metabolic discrimination systems could be used for the rapids election and classification of useful soybean cultivars." target="_blank" title="Multivariate statistical analysis using FT-IR spectrum data of Soybean Core Collection in Korea 새창에서 열림"> Multivariate statistical analysis using FT-IR spectrum data of Soybean Core Collection in Korea		2024.02.13	9
315		2023	작물기초기반과 서은지 The purpose of this study was to develop fuctional food using Salicornia herbacea L. Nutrition facts, free sugar were analyzed by dried sample and polyphenolic and flavonoid compounds, radical scavenging activity, inflammatory reactivity of the sample were analyzed by 0, 50, 100% prethanol solvent extraction in two location Ganghwa and Kimjea. The Nutrition facts showed 7.2 g/100g of moisture, 27.3 g/100g of Ash, 7.0 g/100g of fat, 1.7 g/100g of protein. The total dietary fiber was the highest fact, showed 56.3 g/100g. The total Mineral content was 5,788 mg/100g in Ganghwa, 9,477mg/100g in Kimjea. Na was high mineral contents in both location. The total free sugar content was 37.7 mg/g in Ganghwa, 10.6 mg/g in Kimjea, glucose and sucrose showed high value. The total polyphenol and flavonoid contents showed high value in 100% prethanol extracted (24.2 µg GAE/ml, 15.45 µg CE/ml), Kimjea was higher than Ganghwa. The DPPH and ABTS radical scavenging activity of 0, 50, 100% prethanol extracted expressed as FSC50 and RC50 values. FSC50 values of DPPH were 0% > 50% > 100%. RC50 values of ABTS were 0% > 50% > 100% The anti- inflammatory effect of the sample showed 100% prethanol extraction has the cytotoxic effects on RAW 264.7 cells.  " target="_blank" title="Determination of Nutrition facts, Anti-oxidation Activity and Inflammatory reactivity of Salicornia herbacea L. by different Solvent Extraction 새창에서 열림"> Determination of Nutrition facts, Anti-oxidation Activity and Inflammatory reactivity of Salicornia herbacea L. by different Solvent Extraction		2024.02.13	5
314		2023	작물기초기반과 서은지 The quality and yield of crops produced by field cultivation are expected to decrease for the recent global climate change caused by high temperature. The plant reproductive stage associated with crop yields is vulnerable to unusal temperatures. This study analyzed the yield properties, antioxidant contents, and pollen viability of ‘Arari’ adzuki bean (Vigna angularis L.) under high temperature stress in a temperature gradient greenhouse which forms 0 to 6°C above the outside in 2022, Miryang, Korea. We divided the growth periods by vegetative, anthesis and mature stage, and the highest period was vegetative stage compared by mean of DMT(daily mean temperature) among vegetative stage (con: 27.5℃, T4: 30.5℃), anthesis stage (con: 24.3℃, T4: 27.0℃), mature stage (con: 21.8℃, T4: 26.4℃). The lowest yield (4.15±3.15 g) was the highest temperature (T4: low, 21.4℃; average, 27.0℃; high, 34.6℃) during the anthesis stage. The growth and yield were good at 40.5℃ in the vegetative stage, but when the average temperature was 27.0℃ or higher, growth was poor and the yields were decreased during the flowering period. Total polyphenol and flavonoid contents were increased and the pollen viability was 40.75% in the whole growth period by high temperature (T4: low, 22.9℃; average, 28.8℃; high, 36.9℃). These results represented that the antioxidant levels increased when the antioxidant component was affected at higher temperatures than normal temperatures and the pollen viability related yield decreased as the temperature increased. Our result are reported as the basic data for field growers and breeding of thermos-tolerance in adzuki beans to prepare for the changeable future climate.  " target="_blank" title="The effects of yield properties, antioxidant contents, and pollen viability of adzuki bean (Vigna angularis L.) response in temperature gradient greenhouse and growth periods 새창에서 열림"> The effects of yield properties, antioxidant contents, and pollen viability of adzuki bean (Vigna angularis L.) response in temperature gradient greenhouse and growth periods		2024.02.13	9
313		2023	작물기초기반과 서미숙     주요 식량작물인 콩(Glycine max (L.) Merr.)은 식용 외에 사료용, 녹비용 등 다양한 용도로 재배되고 있다. 최근, 기후변화와 국제 분쟁으로 인해 식량안보의 중요성이 대두되고 있으며, 콩에서도 자급률 향상 및 새로운 용도 창출을 위해 다양한 콩 품종 개발이 요구되고 있다. 유전자교정 기술은 돌연변이 모사 기술로써 GMO를 대체할 기술로 각광 받고 있으며, 콩에서도 유전자교정 기술을 통한 새로운 육종 소재 개발 연구가 활발하게 이루어지고 있다. 본 연구에서는 콩에 유전자교정 기술을 적용하기 위해 필수적인 형질전환 기술을 개발하고, 국내 연구진에게 개발된 형질전환 기술을 안정적으로 제공하고자 하였다. 현재, 지방산에 관여하는 multiple 유전자 4종의 gRNA를 포함하는 CRISPR/Cas9 벡터에 대한 콩 형질전환을 실시하여, 광안콩과 maverick에서 다수의 형질전환 식물체를 생산할 수 있었고, deep sequencing을 통해 유전자교정 효율이 확인되었다. 또한, 제초제 저항성 유전자교정 콩 개발을 위해 형질전환을 실시하여, 유전자의 삽입이 확인된 식물체를 생산하였다. 형질전환을 식물체에서 trans-clean mutant의 신속한 선발과 고정을 통한 유전자교정 식물체의 조기 실용화를 위해 speed breeding 시스템을 이용한 빠른 세대 진전이 필요할 것이다. 본 연구에서는 콩에서 speed breeding 시스템을 구축하고, 최적 조건을 설정하고자 실험을 수행하였다. LED 광량, 토양조건 및 품종에 따른 재배 조건을 검토한 결과, 품종에 따라 다소의 차이는 있으나, 파종 후 평균 23~28일에서 개화가 관찰되었고, 70~80일 후 종자의 수확이 가능하였다. 향후, 광 파장, 재배온도 및 광주기 등 최적의 재배 조건을 검토하여, 유전자교정 작물 개발을 위한 기반을 마련하고자 한다." target="_blank" title="콩 유전자교정 작물 개발을 위한 기반 구축 새창에서 열림"> 콩 유전자교정 작물 개발을 위한 기반 구축		2024.02.13	22
312		2023	작물기초기반과 김신화 땅콩 흰비단병은 토양전염성 병으로 감염된 식물체는 지제부가 수침상으로 무르고, 시들며 결국 말라 죽는다. 한번 발생한 포장에서 연작할 경우, 계속해서 발생하는 경향이 크고 방제도 어렵다. 본 연구에서는 토양전염성 병인 땅콩 흰비단병에 대해 다양한 살균제의 방제 효과를 비교하였다. 연구에 사용한 살균제는 Pencycuron(a.i. 20%, SC), Fluxapyroxad(a.i. 15.3%, SC), Pyraclostrobin(a.i. 11%, SC), Metconazole(a.i. 20%, SC). Tebuconazole(a.i. 20%, SC), Propiconazole(a.i. 1%, G), Captan(a.i. 50, WP), Boscalid+Pyraclostrobin(a.i. 13.6%+6.8%, SC) 8종으로 약제 농도는 약제별 추천 약량의 반량, 정량, 배량을 처리하였다. 연구에 사용한 균주는 2019년부터 2022년까지 수집한 66개의 균주 중 15개 땅콩 품종에 대해서 병원성 검정 결과에 기반하여 병원성이 강한 19SR24를 사용하였다. 땅콩 흰비단병의 살균제 효 과 비교 실험은 온도 30℃, 습도 80~100%의 조건에서 실시하였다. 실험에 사용한 땅콩(품종: 신팔광)은 지름 10cm인 포트(pot)에 파종한 후, 4엽기까지 재배하였다. 병원균 접종 1일 전과 1일 후에 약제를 추천 약량의 반량, 정량, 배량으로 농도를 맞추어 포트당 10ml씩 토양에 관주하였다. 접종방법과 검정조건은 PDA배지에 25℃ 조건으로 10일간 배양한 Agroathelia rolfs의 균총을 잘게 다져 포트당 10g씩 지제부에 접종하였으며, 접종 후 온도 30℃, 습도 90% 이상으로 유지하며 병 발생 억제 효과를 조사하였다. 그 결과, 접종 전과 후 처리 모두 Fluxapyroxad의 병 방제 효과가 75, 66.7%로 가장 우수하였다. Metconazole의 경우 접종 후 약제처리보다 접종 전 약제처리에서 병 방제 효과가 우수하였지만, 땅콩의 생장 이 저해되었다. Tebuconazole의 경우도 정량과 배량농도의 접종 전 약제처리구에서 병 방제 효과가 66.7, 75%로 우수하였으나 땅콩 의 생장이 저해되었다. 이러한 결과를 종합하여 보 았을 때, 땅콩 흰비단병은 발병 전 Fluxapyroxad를 토양관주처리한 것이 가장 병 발생 억제 효과가 우수하였다." target="_blank" title="땅콩 흰비단병 (Agroathelia rolfsii) 살균제 방제 효과 비교 새창에서 열림"> 땅콩 흰비단병 (Agroathelia rolfsii) 살균제 방제 효과 비교		2024.02.13	6
311		2023	작물육종과 이창현 To develop a barley variety suitable for making clear sikhye (traditional Korean rice beverage) with high enzyme activity and low proanthocyanidin content, artificial crossbreeding was conducted between ‘Topgolbori(IT56909)’ which has lodging resistance and high yield, and ‘Ant17-154’, which has low proanthocyanidin content. Subsequently, in 2010, the progeny was crossed again with ‘Hyemi’. After the F1 generation was produced in 2011, the F2-F3 generations were developed as a bulk from 2012 to 2013. In 2014-2015, elite lines with low proanthocyanidin content were selected from the F4-F5 generation through vanillin analysis. In 2016, the selected elite lines were tested as Observational yield trial(OYT), and the ‘IC10008-B-B-9-1’ line was selected. This selected line was recognized for its excellence after a two-years yield trial(2016-2017) and given the breeding line name “Jeonju518”. The regional yield trials(RYT) were conducted for ‘Hyemalgeun’ as a breeding line ‘Jeonju518“ in five different regions from 2019 to 2021(RDA)." target="_blank" title="‘Hyemalgeun’: A new Covered Barley Variety with Low Proanthocyanidin Content Suitable for Making Clear Sikhye 새창에서 열림"> ‘Hyemalgeun’: A new Covered Barley Variety with Low Proanthocyanidin Content Suitable for Making Clear Sikhye		2024.02.13	10
310		2023	작물육종과 이창민 'Chamjinmi' is a mid-maturing rice variety that can be harvested faster than mid-late maturing varieties and also exhibits good the rate of milling, and the taste. From the analysis of the glossiness and taste of cooked rice of Korean native rice resources, we selected the promising resource 'Hoengseongaengmi3', which had better rice eating quality compared to Korean (Ilpum) and Japanese (Hitomebore) varieties. 'Jeonju 654', which was bred by crossing 'Hwayeong' and 'Hoengseongaengmi 3', was developed through pedigree breeding, yield trials, and regional adaptability test, and 'Jeonju 654' was released as 'Chamjinmi' in 2022. The average heading date of 'Chamjinmi' for ordinary planting and late planting for double cropping was August 10th and August 20th, which were 4 days earlier than that of the check variety 'Nampyeong'. The milled rice yield of 'Chamjinmi' was 5.67 MT/ha for ordinary planting (103% of 'Nampyeong') and 5.64 MT/ha for late planting for double cropping (96% of 'Nampyeong'). 'Chamjinmi' had a culm length of 78 cm, a panicle length of 21 cm and 13 panicles per plant. The brown rice type of 'Chamjinmi' was slightly bigger than 'Nampyeong', with a 1,000 grain weight of 23.1 g and a length/width ratio of 1.84. The milled rice of 'Chamjinmi' was translucent, with a protein content of 5.9% and an amylose content of 17.2% (1.3% lower than 'Nampyeong'). The release of 'Chamjinmi' is anticipated to contribute a beneficial mid-maturing rice variety that can be employed across diverse cropping systems in paddy fields, effectively enhancing farm work efficiency.  " target="_blank" title="A new mid maturing and high-eating quality japonica rice variety ‘Chamjinmi’ derived from Korea native resources 새창에서 열림"> A new mid maturing and high-eating quality japonica rice variety ‘Chamjinmi’ derived from Korea native resources		2024.02.13	5
309		2023	작물육종과 서정환 The flag leaf of rice supplies carbon assimilates to the panicle during the ripening stage. The high chlorophyll content of flag leaf under low nitrogen conditions could increase photosynthesis and it is one of the source traits that affect yield in rice. RIL population consists of 88 lines derived from a cross between two Korean japonica, JJ625LG (large grain breeding line) and Namchan (high- yielding variety under low nitrogen conditions). Two parents and 88 RILs were planted by two replications at the paddy field treated with low nitrogen fertilizer (4.5kg/10a). The chlorophyll content of flag leaves was collected at the full flowering stage using SPAD-502 plus. The genotyping of RILs was conducted using Target Capture Sequencing, and 511 informative SNPs were obtained. JJ625LG showed higher Chlorophyll contents of flag leaf and earlier heading date than Namchan. The average and range for chlorophyll contents of RILs were 39.1 and 35.2~44.1, respectively. The linkage map of RILs was constructed using 521 SNPs and represented 1841.3 cM for the total genetic length. Four QTLs for chlorophyll content were detected on chromosomes 1 and 8, explaining 70.7% of phenotypic variation. All alleles derived from JJ625LG showed a positive effect and qCCF1.1 presented the highest LOD, PVE, and additive effect among four QTLs. Besides, three heading date QTLs were identified on chromosomes 1, 2, and 8. qDTH8 was collocated with the same marker interval of qCCF8, and qDTH1 was mapped on 13 cM upstream of qCCF1.2. This result implies chlorophyll content could be related to the heading date in this population. QTLs identified in this study would be applied to improve yield potential under low nitrogen conditions." target="_blank" title="QTL analysis for chlorophyll content under low nitrogen conditions in RILs derived from Korean japonica rice to improve yield potential 새창에서 열림"> QTL analysis for chlorophyll content under low nitrogen conditions in RILs derived from Korean japonica rice to improve yield potential		2024.02.13	9